Urban mosquitoes, particularly Aedes aegypti and Aedes albopictus, are key vectors of diseases like dengue, chikungunya, Zika, and yellow fever. With their ability to thrive in small, water-filled containers around homes, these mosquitoes pose a significant challenge to public health. Adding to the concern is the emergence of Anopheles stephensi, a container-breeding malaria vector spreading from Asia into Africa. This highlights the urgent need for innovative, environmentally sustainable, and scalable mosquito control strategies.
One of the most promising developments is the lethal ovitrap—a device designed to exploit mosquitoes’ natural egg-laying behavior to reduce populations. Over the past few decades, lethal ovitraps have undergone remarkable innovation, evolving into more efficient, cost-effective tools for vector control.
Here’s a look at their journey and potential impact. Early Concepts: Making Containers Lethal
Mass ovitraps emerged as a solution, using small, dark cups with water and organic attractants to lure female mosquitoes. Eggs laid on removable substrates were collected and destroyed. This approach demonstrated potential but required *frequent servicing and was often limited by alternative breeding sites.
Key Innovations in Lethal Ovitrap Design
1. Mass Ovi-Trapping
The Mass Ovi-Trapping (MO) developed in 1966 by Fay and Eliason builds on the concept of ovicups, originally designed to detect the presence of Aedes aegypti mosquitoes during eradication campaigns in the Americas.
This innovative technique involves placing small, dark containers (commonly black or red, made of plastic, metal, or glass) filled with water or organic-infused water across an area to attract ovipositing mosquitoes. Inside, a collection substrate like a wooden tongue depressor or germination paper lines the cup to capture mosquito eggs.
The eggs are then gathered, and the substrate is safely removed and destroyed, effectively interrupting the mosquito life cycle. By deploying ovitraps at scale, this approach helped reduce mosquito populations.
While effective in breaking the mosquito life cycle, this approach required frequent maintenance and struggled in areas with alternative breeding sites. Nevertheless, it established the importance of targeting mosquitoes at the egg-laying stage.

2. Lok Autocidal Ovitrap (LAO)
The Lok Autocidal Ovitrap (LAO), introduced in the 1970s, was a groundbreaking invention aimed at disrupting mosquito breeding cycles. This trap utilized a mechanical design that effectively targeted mosquito eggs. Female mosquitoes were drawn to the trap to lay their eggs, and any larvae that hatched inside were trapped and drowned, preventing them from maturing into adults.
The LAO gained recognition for its success at Singapore’s airport, where it played a significant role in mosquito control efforts. Its environmentally friendly and cost-effective design made it a promising solution for large-scale use. By avoiding harmful chemicals, the LAO aligned with sustainable pest management practices, setting a new standard for innovation at the time.
The trap was not without its limitations however. The mechanical components were prone to failure over time, which reduced its long-term reliability. Additionally, the LAO was unable to target adult mosquitoes, leaving a gap in its overall effectiveness in mosquito population suppression.

3. Zeichner Lethal Ovitrap (ZLO)
The Zeichner Lethal Ovitrap (ZLO) was a significant advancement in mosquito control, designed to overcome the limitations of earlier traps like the Lok Autocidal Ovitrap (LAO).
The trap was developed in the early 2000s by Dr. Brian Zeichner at the US Army Aberdeen Proving Ground. The ZLO was designed to attract and kill egg-laying female mosquitoes, particularly Aedes aegypti and Aedes albopictus.
The ZLO featured a black plastic cup equipped with an insecticide-treated strip strategically placed inside. This strip targeted adult mosquitoes directly, killing them upon contact, while also addressing mosquito larvae by dissolving insecticide into the water, effectively breaking the mosquito life cycle.
Field trials in Brazil, Thailand, and Australia demonstrated the ZLO’s efficacy in significantly reducing mosquito populations. Its ability to simultaneously eliminate adult mosquitoes and larvae made it a comprehensive tool in mosquito management programs.
The ZLO’s design incorporated biodegradable materials and environmentally friendly attractants, increasing its sustainability and appeal for widespread use. By balancing effectiveness with environmental responsibility, the ZLO set a benchmark for innovation in mosquito control technologies, offering a versatile and scalable solution to combat mosquito-borne diseases.
4. Sticky Ovitrap (SO) and Autocidal Gravid Ovitrap (AGO)
The Sticky Ovitrap (SO), developed in the 1990s, introduced a non-chemical approach to mosquito control by using glue to trap gravid female mosquitoes (those ready to lay eggs). It served as a foundational tool for controlling mosquito populations and monitoring vector species such as Aedes aegypti and Aedes albopictus.
Building on the principles of the Sticky Ovitrap, the Autocidal Gravid Ovitrap (AGO) was developed by the Centers for Disease Control and Prevention (CDC) in the early 2000s. This design enhanced trapping efficiency with the addition of a screened water reservoir and adhesive panels, creating a highly effective method for both mosquito surveillance and control.
The AGO targets egg-laying female mosquitoes by luring them to a water-filled container. Once inside, the mosquitoes are trapped and die, effectively reducing breeding populations. In mass deployments, such as those conducted in Puerto Rico, AGO traps achieved an impressive 88% reduction in mosquito populations and significantly decreased exposure to the chikungunya virus.

5. Gravid Aedes Trap (GAT)
Developed in the 2010s, the Gravid Aedes Trap (GAT) represents a significant advancement in mosquito control technology. It features a 1.2L matte black plastic bucket with a distinctive black entry funnel, meticulously designed to prevent mosquito escape. Inside, a water infusion mimics stagnant water, using its enticing odor to attract egg-laying female mosquitoes.
Although the GAT’s monthly maintenance requirement was a step backward compared to earlier designs, its ability to target and eliminate both adult mosquitoes and larvae made it an indispensable tool in urban vector control strategies.

6. Innovations in Autodissemination
The autodissemination approach, introduced in the 2010s, marked a significant shift from traditional traps by enabling mosquitoes to spread control agents to breeding sites. These devices contaminate adult mosquitoes with fungal biopesticides, killing female mosquitoes in one to three weeks and allowing them time to disseminate IGRs to nearby breeding areas.
While effective, the technology comes with challenges. The trap requires regular maintenance and part replacement. The fungal biopesticides require temperature-controlled storage, complicating transportation logistics. Additionally, technicians handling these traps must use personal protective equipment (PPE) to prevent inhalation of the fungal spores.

7. Dual-Action Lethal Ovitrap (INZECTO Trap)
The Dual-Action Lethal Ovitrap (INZECTO), introduced in 2021 following a decade of research and development at the University of Florida , is a mosquito trap that employs two complementary mechanisms to effectively target and eliminate mosquitoes.
The INZECTO Mosquito Trap, building on the strengths of its predecessors, employs a unique polymer coating infused with both an adulticide (permethrin) and a larvicide (pyriproxyfen) to target adult mosquitoes and their offspring simultaneously.
Unlike autodissemination traps, the INZECTO Trap ensures consistent performance in all weather conditions, providing year-round reliability. The simplicity of the device requires one-part and one-step (just add water) for deployment.
Designed for durability, the polymer coating resists deterioration in extreme temperatures, whether hot 🌡or cold ❄️, making it a reliable solution in any climate.
Unlike maintenance-intensive options like GAT or autodissemination traps, the INZECTO Trap delivers long-lasting results with minimal upkeep, remaining effective for up to three months. Female mosquitoes prefer to lay eggs in the INZECTO Trap resulting in a 82 – 94% reduction of eggs in other competing containers.

The Future of Lethal Ovitraps
As the fight against mosquito-borne diseases continues, the evolution of lethal ovitraps highlights the importance of innovation in pest management. These traps offer a sustainable alternative to traditional chemical sprays, with reduced environmental impact and improved cost-efficiency. While the INZECTO trap sets a high standard, future developments will likely integrate new materials, attractants, and monitoring technologies to enhance effectiveness.
Lethal ovitraps are not just tools—they are a testament to the power of science and innovation in addressing global health challenges.
The progression from Mass Ovi-Trapping to the INZECTO Mosquito Trap underscores the relentless drive for innovation in mosquito control. By continuously integrating new materials, attractants, and automation, the next generation of ovitraps promises even greater efficiency and scalability.
If you’re a public health professional, pest control expert, or researcher in the field of vector management, now is the time to explore these cutting-edge technologies and consider how they can play a pivotal role in your strategies.
Connect with us to learn more about how lethal ovitraps like the INZECTO Trap can help you achieve more effective, environmentally friendly mosquito control. Together, we can make a lasting impact on global health.